rabbit anti rad18 polyclonal antibody  (Proteintech)

Journal: Medicine

Article Title: Risk of cervical squamous cell carcinoma associated with a single nucleotide polymorphism in the RAD18 gene in the Chinese population and its significance as a predictive biomarker

doi: 10.1097/MD.0000000000044017

Figure Lengend Snippet: RAD18 expression in CSCC with different genetic polymorphisms as determined by immunohistochemistry (40× objective, magnified). (A) rs250403-AA; (B) rs250403-AG; (C) rs250403-GG; (D) rs615967-AA; (E) rs615967-AG; (F) rs615967-GG. The distinct brown coloration is mainly located in the nucleus of the positive cells.

Article Snippet: The sections were firstly incubated with Rabbit anti-RAD18 Polyclonal antibody (1:200, 18333-1-AP, Proteintech), and then incubated with Dako Envision TM Peroxidase (Dako Diagnostica, Hamburg, Germany), and visualized with 3,3’-diaminobenzidine tetrahydrochloride (Dako).

Techniques: Expressing, Immunohistochemistry

Journal: Cell reports

Article Title: Fanconi anemia-associated chromosomal radial formation is dependent on POLθ-mediated alternative end joining

doi: 10.1016/j.celrep.2023.112428

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-RAD18 , Bethyl Laboratories , Cat # A301–340A; RRID: AB_937974.

Techniques: Recombinant, Membrane, Plasmid Preparation, Staining, Transfection, Reverse Transcription, Software, Real-time Polymerase Chain Reaction, Microscopy, Cell Counting

Journal: Molecular Cell

Article Title: The ubiquitin ligase RFWD3 is required for translesion DNA synthesis

doi: 10.1016/j.molcel.2020.11.029

Figure Lengend Snippet: RFWD3 ubiquitin ligase activity regulates PCNA ubiquitylation in human cells (A) U2OS cells or U2OS cells expressing Strep-HA-PCNA were transfected with siCtrl or 4 different siRNAs against RFWD3 and either left untreated or treated with UV (30 J/m 2 ). PCNA was recovered under denaturing conditions via Strep-Tactin pull-down and analyzed by immunoblotting with the indicated antibodies. (B) U2OS cells or U2OS/FRT GFP-RFWD3 WT or catalytic inactive (C315A) cells were treated with the indicated siRNAs, transfected with either empty vector (EV) or Strep-HA-PCNA plasmids, and treated with doxycycline to induce expression of RFWD3. UV treatment and protein pull-down were performed as in (A). (C) U2OS cells were transfected with the indicated plasmids and then subjected to Strep-Tactin pull-down as in (A). (D) U2OS/FRT GFP-RFWD3 WT cells were treated with doxycycline and transfected with EV or Strep-HA-PCNA 24 h before lysis in denaturing buffer. Lysates were subjected to Strep-Tactin pull-down in denaturing conditions, washed, and incubated with USP2 (ubiquitin protease) and/or UPL1 (SUMO protease), as indicated. (E) U2OS cells or U2OS/FRT GFP-RFWD3 WT cells were transfected with either control (siCtrl) or RAD18 siRNAs. After 48 h, cells were transfected with either EV or Strep-HA-PCNA plasmids and treated with doxycycline to induce the expression RFWD3 WT. Then, cells were treated with UV for 4 h and processed for Strep-Tactin pull-down as described in (A). The asterisk denotes a non-specific band.

Article Snippet: Rabbit polyclonal antibody anti RAD18 , Bethyl Laboratories , Cat#A301-340A; RRID: AB_937974.

Techniques: Ubiquitin Proteomics, Activity Assay, Expressing, Transfection, Western Blot, Plasmid Preparation, Lysis, Incubation, Control

Journal: Molecular Cell

Article Title: The ubiquitin ligase RFWD3 is required for translesion DNA synthesis

doi: 10.1016/j.molcel.2020.11.029

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal antibody anti RAD18 , Bethyl Laboratories , Cat#A301-340A; RRID: AB_937974.

Techniques: Ubiquitin Proteomics, Recombinant, Mutagenesis, Staining, Magnetic Beads, Transfection, Sequencing, Software